Tobi Amusan, Nigeria's medal hopeful in the women's 100m hurdles, was unable to secure a spot in the final of the event.
Despite being the world record holder, Amusan clocked a time of 12.55 seconds, finishing third in her heat and ninth overall in the semifinals.
The 27-year-old athlete had been in impressive form leading up to the Olympics, recording a season's best and world-leading time of 12.40 seconds (0.9) at the Jamaican Athletics Invitational in Kingston in May
Meanwhile, Nigeria's 4x400m team has now been disqualified for a lane infringement.
The team qualified for the final after finishing 2nd in heat 2 with a Season's Best of 2:59.81.
Nigeria will no longer compete in the final of the men's 4x400m race at the ongoing Paris Olympics games.